|
KMID : 0613820180280060708
|
|
Journal of Life Science
2018 Volume.28 No. 6 p.708 ~ p.717
|
|
|
Antioxidant Activities and Hepato-protective Effects of Stauntonia hexaphylla Fruit Extract Against H2O2-induced Oxidative Stress and Acetaminophen- induced Toxicity
|
|
Lee Gyu-Ok
Kim Jae-Yong
Kang Hu-Wan
Bae Dong-Hyuck
Choi Chul-Yung
|
|
|
Abstract
|
|
|
|
The antioxidant activity and protective effects of a hot water extract from the Stauntonia hexaphylla fruit (WESHF) were investigated in vitro and in vivo. The total polyphenol and flavonoid contents of WESHF were 16.13±0.27 mg gallic acid equivalent/g and 4.7±0.80 mg catechin equivalent/g, respectively. In addition, the DPPH radical-scavenging activity (SC50) and the Oxygen Radical Absorbance capacity of WESHF were 63.62±4.10 μg/ml and 90.63±5.29 μM trolox equivalent/g, respectively. The hepatoprotective effect of WESHF against hydrogen peroxide-induced oxidative damage was investigated. H2O2-induced liver damage on HepG2 cells was prevented by 200 μg/ml of WESHF. Furthermore, to investigate the protection mechanism of WESHF on hydrogen peroxide-induced cytotoxicity in HepG2 cells, pre-treatment with 200 μg/ml of WESHF significantly attenuated a decrease in the activities of CAT, SOD, GR, and GPx. The hepatoprotective activity of WESHF was evaluated in an experimental model of hepatic damage induced by acetaminophen (APAP). The levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were significantly decreased in the livers of mice treated with 200 mg/kg of WESHF compared to the APAP-treated group. The lipid peroxidation level, which increased after APAP administration, was significantly reduced in the WESHF group. In addition, histological examinations of the liver showed the same protective effect of WESHF treatment. Based on these findings, it is suggested that WESHF has potent hepatoprotective effects, and the mechanism that causes this type of protection could be related to antioxidant pathways.
|
|
|
KEYWORD
|
|
|
Acetaminophen, antioxidant, hepatoprotective effect, oxidative stress, Stauntonia hexaphylla
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|